Molecular Structure Explorer
5-Amino-1MQ
Structure of the compound covered in this guide.
Last reviewed: October 2026 · Cleared Peptides research team
Research use only. 5-Amino-1MQ: Research Guide to the NNMT Inhibitor is supplied for laboratory research. It is not a drug, food, cosmetic, or dietary supplement. It is not FDA-approved. It is not for human or veterinary administration. This page does not give a dose or a method of administration. A study cited on this page describes that study’s own test article. It does not make this lot an approved medicine. To check a fact, open the PubMed or DOI link, confirm the authors and year, and match the paper’s compound name, CAS number, or sequence to the specification table. The full method is on the research desk page. Identity and purity for a lot are only what that lot’s certificate of analysis reports. An HPLC percentage is not a sterility result and not an endotoxin result unless the certificate prints those tests.
5-Amino-1MQ (5-amino-1-methylquinolinium) is a small, membrane-permeable quinolinium compound characterized as a selective inhibitor of nicotinamide N-methyltransferase (NNMT), an enzyme that methylates nicotinamide using S-adenosylmethionine.
5-Amino-1MQ Structure and Identifiers
| Name | 5-Amino-1MQ |
|---|---|
| Synonyms | 5-Amino-1-methylquinolinium |
| CAS number | 42464-96-0 |
| PubChem CID | 66522933 |
| Molecular formula | C10H11N2+ (salt form per COA) |
| Molecular weight | ≈159.2 g/mol (cation) |
| Type | Small molecule (not a peptide) |
| Form | Powder |
Why NNMT Matters in Research
NNMT converts nicotinamide to 1-methylnicotinamide, diverting it from the NAD+ salvage pathway and consuming methyl groups. Inhibiting NNMT in cell models is therefore used to study NAD+ levels and methyl-donor balance — often alongside NAD+.
Published In-Vitro Research Areas
- NNMT enzyme-inhibition assays
- NAD+ and SAM metabolite measurements in adipocyte cultures
This summary describes laboratory research only and makes no claim about effects in humans or animals.
Purity Testing and Storage
Every lot of 5-Amino-1MQ supplied by Cleared Peptides is tested by an independent laboratory for identity and purity (or composition, for multi-component preparations), and the lot Certificate of Analysis is published on our COA page. Store 5-Amino-1MQ sealed at −20 °C, dry and protected from light. Handle according to your institution’s chemical-hygiene procedures.
Research materials referenced in this guide
Analytical testing notes for 5-Amino-1MQ
5-Amino-1MQ is identified in this guide as CAS 42464-96-0, formula C10H11N2+ (salt form per COA), listed mass ≈159.2 g/mol (cation). An identity test compares a measurement with those values. A mass spectrum is the usual measurement for a peptide. FTIR, when a certificate uses it, is a supporting infrared fingerprint and is less specific for closely related sequences.
5-Amino-1MQ is not a peptide. Identity and purity may be chromatographic (HPLC or GC) or spectroscopic, depending on the method named on the certificate. The peptide-specific notes below — sequence mass, 214 nm peptide-bond detection, and net peptide content — should not be assumed. Sterility and endotoxin still apply only when those lines are on the report.
Read the method named on the certificate before treating a purity percentage as an HPLC area-percent result. Small molecules in this library may be assessed by HPLC, gas chromatography, or spectroscopy. The instrument named on the report is the one that applies. A purity result still does not measure sterility, endotoxin, or a labeled fill weight.
A sterility test and an endotoxin test are not included in a chemical purity result. The compendial sterility test is a culture method (USP <71>): membrane filtration or direct inoculation into fluid thioglycollate medium and soybean-casein digest medium, incubated for 14 days. A PCR sterility screen uses a thermal cycler to detect microbial DNA and is a different method. The endotoxin test (USP <85> LAL, or USP <86> recombinant Factor C) reports endotoxin units with an incubating reader or a cartridge reader. Use a result only when that method is printed on the lot certificate. One published example in this catalog states that the report does not cover microbial contamination or endotoxins. The full description of equipment, standards, and how US laboratory accreditation works is in peptide analytical testing. Lot records are on the COA page.
Frequently Asked Questions
What enzyme does 5-Amino-1MQ inhibit?
Nicotinamide N-methyltransferase (NNMT).
Is 5-Amino-1MQ for human use?
No. Cleared Peptides supplies 5-Amino-1MQ for laboratory research use only; it is not for human or veterinary use.
Selected References
Peer-reviewed literature on the compounds discussed in this guide, listed as scientific background for laboratory researchers. Citation does not imply endorsement by the authors or any use other than in-vitro research.
- Ruf S, et al. Novel nicotinamide analog as inhibitor of nicotinamide N-methyltransferase. Bioorg Med Chem Lett. 2018;28(5):922-925. PubMed 29433927 · DOI
- Li P, et al. Enhancing nicotinamide N-methyltransferase bisubstrate inhibitor activity through 7-deazaadenosine and linker modifications. Bioorg Chem. 2024;143:106963. PubMed 38048700 · DOI
- Akar S, et al. Small molecule inhibitor of nicotinamide N-methyltransferase shows anti-proliferative activity in HeLa cells. J Obstet Gynaecol. 2021;41(8):1240-1245. PubMed 33645410 · DOI
Laboratory note: solubility depends on salt form and solvent. This guide does not state a single solubility figure. Handling of lyophilized material is described in the peptide storage guide.
Research Use Only Notice
This guide is provided for educational and laboratory reference purposes. It summarizes published, primarily in-vitro and structural research literature and does not describe, recommend or imply any use in humans or animals. Products sold by Cleared Peptides are for laboratory research use only; they are not drugs, foods, cosmetics or dietary supplements, are not FDA-approved, and are not intended to diagnose, treat, cure, mitigate or prevent any disease. No dosing, administration or therapeutic information is provided.
